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rabbit anti total 4ebp1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti total 4ebp1
    Rabbit Anti Total 4ebp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+4ebp1/Phospho-4E-BP1+(Thr37%2F46)+Antibody/pm41832996-38-143-146
    Average 96 stars, based on 1246 article reviews
    rabbit anti total 4ebp1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: The COPD GWAS gene ADGRG6 instructs function and injury response in human iPSC-derived type II alveolar epithelial cells.
    Article Snippet: The following antibodies were used in this study: Total 4EBP1 (Cell Signaling Technology #9644), Non-Phospho-4EBP1 (Thr46) (Cell Signaling Technology #4923), total Rb (Cell Signaling Technology #9309), Phospho-Rb (Cell Signaling Technology #8516), and GAPDH (Invitrogen AM4300).

    Article Title: Aprepitant inhibits the progression of esophageal squamous cancer by blocking the truncated neurokinin‑1 receptor
    Article Snippet: Meanwhile, PI3k-p110α (cat. no. 4255), total AKT (cat. no. 9272), phospho-AKT (cat. no. 4060), total mTOR (cat. no. 2983), phospho-mTOR (cat. no. 5536), total 4EBP1 (cat. no. 9644), phospho-4EBP1 (cat. no. 9451), total p70S6K (cat. no. 2708) and phospho-p70S6K (cat. no. 9234) were purchased from Cell Signaling Technology, Inc. Membranes were then washed with tris-buffered saline Tween (1% Tween-20) and incubated with secondary HRP-conjugated antibodies (1:10,000) for 2 h at room temperature.

    Article Title: The COPD GWAS gene ADGRG6 instructs function and injury response in human iPSC-derived type II alveolar epithelial cells
    Article Snippet: The following antibodies were used in this study: Total 4EBP1 (Cell Signaling Technology #9644), Non-Phospho-4EBP1 (Thr46) (Cell Signaling Technology #4923), total Rb (Cell Signaling Technology #9309), Phospho-Rb (Cell Signaling Technology #8516), and GAPDH (Invitrogen AM4300).

    Incubation:

    Article Title: Treatment with L-type amino acid transporter 1 inhibitor JPH203 enhances protein synthesis in C2C12 myotubes
    Article Snippet: The separated proteins were transferred to polyvinylidene difluoride membranes, and the membranes were blocked with Bullet Blocking One for Western Blotting (13779-01, Nacalai Tesque) for 5 min at room temperature. .. The membranes were then incubated overnight at 4 °C with following primary antibodies: puromycin (1:3000, MABE343, Merck Millipore, Darmstadt, Germany), phospho-p70S6K (1:1000, Thr389, #9205, Cell Signaling Technology (CST), Danvers, MA, USA), total-p70S6K (1:1000, #2708, CST), phsopho-rpS6 (1:3000, Ser240/244, #5364, CST), total-rpS6 (1:3000, #2217, CST), phospho-4EBP1 (1:1000, Thr37/46, #2855, CST), total-4EBP1 (1:1000, #9466, CST), phospho-Akt (1:1000, Ser473, #9271, CST), total-Akt (1:1000, #9272, CST), Atrogin-1 (1:3000, ab168372, Abcam, Cambridge, UK), MuRF-1 (1:1000, sc-398608, Santa Cruz Biotechnology, TX, USA), K48 linkage-specific polyubiquitin (1:1000, #8081, CST), phospho-ULK1 (1:1000. .. Ser757, #14202, CST; 1:1000, Ser555, #5869, CST), total-ULK1 (1:1000, #8054, CST), p62/SQSTM (1:3000, PM045, Medical & Biological Laboratory, Nagoya, Japan), or LC3 (1:2000, #2775, CST).

    Article Title: Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
    Article Snippet: .. After 5 min blocking with Bullet Blocking One for Western Blotting (13779‐01, Nacalai Tesque, Kyoto, Japan) at room temperature, the membranes were incubated overnight at 4°C with following primary antibodies: GFP (#2956, Cell Signaling Technology), platelet‐derived growth factor receptor alpha (PDGFRα, #3174, Cell Signaling Technology), phosphor‐Akt (Ser473, #9275, Cell Signaling Technology), total‐Akt (#4691, Cell Signaling Technology), phosphor‐p70S6K (Thr389, #9234, Cell Signaling Technology), total‐p70S6K (#2708, Cell Signaling Technology), phosphor‐rpS6 (Ser240/244, #2215, Cell Signaling Technology), total‐rpS6 (#2217, Cell Signaling Technology), phosphor‐4EBP1 (Thr37/46, #9459, Cell Signaling Technology), total‐4EBP1 (#9644, Cell Signaling Technology), ubiquitinated proteins (#3936, Cell Signaling Technology), K48‐linkage‐specific polyubiquitin (#8081, Cell Signaling Technology), Atrogin‐1 (ab168372, Abcam, Cambridge, UK), MuRF‐1 (sc‐398608, Santa Cruz Biotechnology, Dallas, TX, USA), LC3 (#2775, Cell Signaling Technology), p62/SQSTM (PM045, Medical & Biological Laboratory, Nagoya, Japan), phosphor‐ULK1 (Ser757, #14202, Cell Signaling Technology; Ser555, #5869, Cell Signaling Technology), total‐ULK1 (#8054, Cell Signaling Technology), IGFBP3 (10189‐2‐AP, Proteintech, Rosemont, IL, USA), or IGFBP5 (55205‐1‐AP, Proteintech). .. After washing, membranes were incubated with the appropriate secondary antibodies (#7074 or #7076, Cell Signaling Technology) for 1 h at room temperature.

    Article Title: Treatment with L-type amino acid transporter 1 inhibitor JPH203 enhances protein synthesis in C2C12 myotubes.
    Article Snippet: The separated proteins were transferred to polyvinylidene difluoride membranes, and the membranes were blocked with Bullet Blocking One for Western Blotting (13779- Scientific Reports | (2025) 15:40805 3| https://doi.org/10.1038/s41598-025-24534-2 01, Nacalai Tesque) for 5 min at room temperature. .. The membranes were then incubated overnight at 4 °C with following primary antibodies: puromycin (1:3000, MABE343, Merck Millipore, Darmstadt, Germany), phosphop70S6K (1:1000, Thr389, #9205, Cell Signaling Technology (CST), Danvers, MA, USA), total-p70S6K (1:1000, #2708, CST), phsopho-rpS6 (1:3000, Ser240/244, #5364, CST), total-rpS6 (1:3000, #2217, CST), phospho-4EBP1 (1:1000, Thr37/46, #2855, CST), total-4EBP1 (1:1000, #9466, CST), phospho-Akt (1:1000, Ser473, #9271, CST), total-Akt (1:1000, #9272, CST), Atrogin-1 (1:3000, ab168372, Abcam, Cambridge, UK), MuRF-1 (1:1000, sc-398608, Santa Cruz Biotechnology, TX, USA), K48 linkage-specific polyubiquitin (1:1000, #8081, CST), phospho-ULK1 (1:1000. .. Ser757, #14202, CST; 1:1000, Ser555, #5869, CST), total-ULK1 (1:1000, #8054, CST), p62/SQSTM (1:3000, PM045, Medical & Biological Laboratory, Nagoya, Japan), or LC3 (1:2000, #2775, CST).

    Blocking Assay:

    Article Title: Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
    Article Snippet: .. After 5 min blocking with Bullet Blocking One for Western Blotting (13779‐01, Nacalai Tesque, Kyoto, Japan) at room temperature, the membranes were incubated overnight at 4°C with following primary antibodies: GFP (#2956, Cell Signaling Technology), platelet‐derived growth factor receptor alpha (PDGFRα, #3174, Cell Signaling Technology), phosphor‐Akt (Ser473, #9275, Cell Signaling Technology), total‐Akt (#4691, Cell Signaling Technology), phosphor‐p70S6K (Thr389, #9234, Cell Signaling Technology), total‐p70S6K (#2708, Cell Signaling Technology), phosphor‐rpS6 (Ser240/244, #2215, Cell Signaling Technology), total‐rpS6 (#2217, Cell Signaling Technology), phosphor‐4EBP1 (Thr37/46, #9459, Cell Signaling Technology), total‐4EBP1 (#9644, Cell Signaling Technology), ubiquitinated proteins (#3936, Cell Signaling Technology), K48‐linkage‐specific polyubiquitin (#8081, Cell Signaling Technology), Atrogin‐1 (ab168372, Abcam, Cambridge, UK), MuRF‐1 (sc‐398608, Santa Cruz Biotechnology, Dallas, TX, USA), LC3 (#2775, Cell Signaling Technology), p62/SQSTM (PM045, Medical & Biological Laboratory, Nagoya, Japan), phosphor‐ULK1 (Ser757, #14202, Cell Signaling Technology; Ser555, #5869, Cell Signaling Technology), total‐ULK1 (#8054, Cell Signaling Technology), IGFBP3 (10189‐2‐AP, Proteintech, Rosemont, IL, USA), or IGFBP5 (55205‐1‐AP, Proteintech). .. After washing, membranes were incubated with the appropriate secondary antibodies (#7074 or #7076, Cell Signaling Technology) for 1 h at room temperature.

    Western Blot:

    Article Title: Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
    Article Snippet: .. After 5 min blocking with Bullet Blocking One for Western Blotting (13779‐01, Nacalai Tesque, Kyoto, Japan) at room temperature, the membranes were incubated overnight at 4°C with following primary antibodies: GFP (#2956, Cell Signaling Technology), platelet‐derived growth factor receptor alpha (PDGFRα, #3174, Cell Signaling Technology), phosphor‐Akt (Ser473, #9275, Cell Signaling Technology), total‐Akt (#4691, Cell Signaling Technology), phosphor‐p70S6K (Thr389, #9234, Cell Signaling Technology), total‐p70S6K (#2708, Cell Signaling Technology), phosphor‐rpS6 (Ser240/244, #2215, Cell Signaling Technology), total‐rpS6 (#2217, Cell Signaling Technology), phosphor‐4EBP1 (Thr37/46, #9459, Cell Signaling Technology), total‐4EBP1 (#9644, Cell Signaling Technology), ubiquitinated proteins (#3936, Cell Signaling Technology), K48‐linkage‐specific polyubiquitin (#8081, Cell Signaling Technology), Atrogin‐1 (ab168372, Abcam, Cambridge, UK), MuRF‐1 (sc‐398608, Santa Cruz Biotechnology, Dallas, TX, USA), LC3 (#2775, Cell Signaling Technology), p62/SQSTM (PM045, Medical & Biological Laboratory, Nagoya, Japan), phosphor‐ULK1 (Ser757, #14202, Cell Signaling Technology; Ser555, #5869, Cell Signaling Technology), total‐ULK1 (#8054, Cell Signaling Technology), IGFBP3 (10189‐2‐AP, Proteintech, Rosemont, IL, USA), or IGFBP5 (55205‐1‐AP, Proteintech). .. After washing, membranes were incubated with the appropriate secondary antibodies (#7074 or #7076, Cell Signaling Technology) for 1 h at room temperature.



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